Fig. 1

Ehmt2 is dispensable for normal muscle development. a Ehmt2 deletion efficiency as measured by functional allele frequency in FACS-purified satellite cells. Analysis by gDNA allele counting using ddPCR, n ≥ 3. b Relative abundance of H3K9me2 in whole skeletal muscle tissue lysate of wildtype and knockout mice, normalized to histone H3. c Immunofluorescence detection of EHMT2 on myofiber. d Number of live births from n ≥ 3 mating pairs of Myod ^Cre Ehmt2 ^{floxed/floxed} mice. e, f Neonatal weight and growth curve of wildtype and knockout mice at D₀ − D₁. g Masson’s trichrome stain of histological sections of the tibialis anterior muscle of adult mice. h Whole muscle weight. i Myofiber size measurement by cross-sectional area