Fig. 4
Western blot analysis of CaV1.1 protein levels in symptomatic SOD1G93A skeletal muscle. a western blots of lysates obtained from four different tibialis anterior (b) gastrocnemius muscles of age-matched wild-type and symptomatic SOD1G93A mice are shown (N = 4 for both groups). In each case, the blots were probed with a monoclonal antibody directed to CaV1.1 (top panels), then stripped and reprobed sequentially for α-actin (middle panels) and then histone H3 (bottom panels). A protein ladder standard resides in lane 1 of each blot; molecular weights are indicated in kDa. c A western blot of lysates obtained from FDB muscles of age-matched wild-type and symptomatic SOD1G93A mice are shown (N = 2 for both groups). The blot was first probed with a monoclonal antibody directed to CaV1.1 (top panel) and then stripped and reprobed sequentially with antibodies directed to α-actin (middle panel) and then histone H3 (bottom panel). For each blot, changes in expression were determined by comparing the intensity of each CaV1.1 band in the top panel to the corresponding actin band the middle panel. For each panel, similar results were observed in at least three experiments