Fig. 2
From: Characterization of a Dmd EGFP reporter mouse as a tool to investigate dystrophin expression
Correct localization of the EGFP-tagged dystrophin at the sarcolemma. Immunofluorescent staining of cryosections from the tibialis anterior of Dmd EGFP mice with antibodies against the FLAG-tag, C-terminal dystrophin (Dys2), rod-domain dystrophin (MANDYS19), cytoskeletal β-spectrin, and the basement membrane protein laminin (all colored in red). Exact colocalization was observed between the natural EGFP fluorescence (green) and the signals deriving from the two anti-dystrophin antibodies. Merged images were counterstained with DAPI (blue)