Fig. 3

Dmd ^EGFP mice do not show signs of muscle dystrophy. a Hematoxylin and eosin (H&E) staining of tibialis anterior (TA) muscle sections from adult (8- to 12-week-old) and aged (10-month-old) wild-type (WT) and Dmd ^EGFP mice show normal morphology. For comparison, H&E staining of an aged-matched mdx TA muscle shows the morphological characteristics of muscle dystrophy comprising a large variation of fiber size, mononuclear cell infiltrates, fibrosis, and abundant centrally located myonuclei. b Wild-type and Dmd ^EGFP mice show normal localization and expression of the DAPC components α-dystroglycan, γ-sarcoglycan, and nNOS in TA muscle cross sections. c Serum creatine phosphokinase (CPK) activities in the serum of wild-type, Dmd ^EGFP, and mdx mice (n = 3 of each genotype). Values are shown as means ± S.E.M., p values were calculated using one-way ANOVA. d The histograms depict the minimal Feret diameter of muscle fibers from EDL muscles (upper panel) and soleus muscles (lower panel) from adult wild-type and Dmd ^EGFP mice. Values are shown as means ± S.E.M. (n = 3 mice from each genotype were analyzed)