Skip to main content
Fig. 7 | Skeletal Muscle

Fig. 7

From: Characterization of a Dmd EGFP reporter mouse as a tool to investigate dystrophin expression

Fig. 7

Dystrophin-EGFP expression of single fibers. a Isolated single EDL muscle fibers from Dmd EGFP mice and wild-type littermates. The natural EGFP fluorescence (green) permits easy distinction between Dmd EGFP and wild-type fibers without necessity for immunostaining. Fibers were fixed and stained with DAPI (blue). b Natural EGFP expression at the neuromuscular junction of a single fiber co-stained with AlexaFluor568 labeled α-bungarotoxin (BTX, red). The higher magnification shows colocalization of natural dystrophin EGFP with the acetylcholine receptor. Fibers were counterstained with DAPI

Back to article page