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Fig. 2 | Skeletal Muscle

Fig. 2

From: p38α MAPK disables KMT1A-mediated repression of myogenic differentiation program

Fig. 2

MKK6EE-p38α activation can abolish KMT1A inhibited gene activation by MyoD. a C2-4RE-luc/KMT1A-F cells were generated by overexpression of Flag-KMT1A in C2-4Re-luc reporter cells by lentiviral delivery. Subsequently, luciferase activity was assessed in C2-4RE-luc and C2-4RE-luc/KMT1A-F cells in GM or DM and expressed protein after normalization as fold activation. Western blot analysis of these reporter cells extracts probed with antibodies to detect MyoD and β-actin for loading control. b Luciferase activity was assessed in C2-4RE-luc/KMT1A-F cells following expression of MKK6EE, MKK6DN or vector control (Em) via lentiviral delivery grown in GM and expressed after protein normalization as fold activation. c C2-4RE-luc/KMT1A-F cells expressing vector control (Em) or MKK6EE via lentiviral delivery were grown in GM or DM and MKK6EE-transduced cells treated with or without SB. Afterward, luciferase activity was assessed in these cells and values expressed after protein normalization. Where appropriate, error bar, ±SEM (n = 3)

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