Fig. 5

p38α unleashes KMT1A via phosphorylation from MyoD during differentiation. a Control IgG or anti-MyoD immunoprecipitates retrieved from cell extracts of C2C12 cells grown GM or DM with or without SB were divided equally and evaluated one part for western blot analysis, probed with antibodies against KMT1A and MyoD. Another part was subjected to HMT activity assay. Methylated and input H3(N) were detected by fluorography and Commassie, respectively. b Control IgG or anti-KMT1A immunoprecipitates retrieved from cell extracts used in a were subjected to western blot analysis, probed with antibodies against MyoD and KMT1A. c Control IgG, anti-KMT1A, or anti-MyoD immunoprecipitates were retrieved from cell extracts of C2C12 cells expressing vector control (Em), MKK6EE or MKK6DN via lentiviral delivery grown in GM or DM. Subsequently, IgG and KMT1A immunoprecipitates were subjected to western blot analysis, probed with antibodies against MyoD and KMT1A. Separately, IgG and MyoD immunoprecipitates were subjected to HMT activity assay. Methylated and input H3(N) were detected by fluorography and Commassie, respectively