Fig. 6

Inhibition of p38α activation retains KMT1A on chromatin of the myogenin promoter during differentiation. a ChIP analysis of E box-containing regions within the Myog and MyHCIIB promoters was performed on chromatin of C2C12 cells gown in GM or DM treated with or without SB. Schematic diagram represents E box-containing regions of these genes promoters and arrows indicate primers used for PCR reaction. ChIP was performed with antibodies against proteins as indicated, where no antibodies or IgG were used as controls. b ChIP analysis of same regions of Myog promoter was performed on chromatin of C2C12 cells expressing vector control (Em) or MKK6EE vial lentiviral delivery grown in GM in the presence or absence of SB. ChIP was performed with anti-H3K9me3 or anti-KMT1A antibodies, where no antibodies or IgG were used as controls