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Fig. 1 | Skeletal Muscle

Fig. 1

From: Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Fig. 1

Six1 proteins are predominantly localized in nuclei of fast type skeletal muscles. a, b Western blot analysis of Six1 proteins in total lysates (a) and in nuclear fractions (b) of GP and SOL muscles of 2-week, 3-week, and 12-week-old animals. Quantification of nuclear Six1 proteins at these developmental stages is presented. Lamin B is used as a nuclear control protein, and α-tubulin as a cytoplasmic control protein. c Immunostaining of Six1 (green), Dystrophin (red), MyHCI (red) Laminin (green) and MY32 (red) in soleus and gastrocnemius (GP) muscles of embryonic E18.5 mouse fetuses. The second image is an enlargement of the yellow square drawn on the first image. White arrows indicate Six1 nuclear staining. d. Immunostaining of Six1 (green), Dystrophin (red), MyCHI (red), MyHCIIA (red) and Laminin (green) in SOL and GP of 20-week-old mice. The lower panels are enlargment of the yellow squares drawn on the top image. White arrows indicate nuclear Six1 detection. Some MyHCI and MyHCIIA positive fibers are indicated as I or IIA, respectively. SOL soleus muscle, GP gastrocnemius plantaris muscle

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