Fig. 2
From: Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle
Six1 deficiency induced a lack of MyHCIIA fibers in soleus. a Immunostaining of MyHCI (red), MyHCIIA (red), MyHCemb (red), and laminine (green) in soleus muscles of 3-week-old control and cSix1 KO mice. SOL soleus, GP gastrocnemius plantaris, F fibula. b Percentage of myofibers expressing MyHCI, MyHCIIA and MyHCemb in soleus of 3-week-old control and cSix1 KO mice, Ctrl (n = 3), cSix1 (n = 3). c Immunostaining of MyHCI (red), MyHCIIA (red), and laminine (green) in soleus of 12-week-old control and cSix1 KO mice. d Percentage of myofibers expressing MyHCI, MyHCIIA in soleus of 12-week-old control and cSix1 KO mice, Ctrl (n = 3), cSix1 (n = 3). e Immunostaining of Six1 (green) and Dystrophin (red) in soleus of 3 weeks old cSix1 KO mice and Ctrl mice. White arrows indicate nuclear Six1 staining. Nuclei are stained with Dapi (blue). *P < 0.05, **P < 0.01, ***P < 0.001