Skip to main content
Fig. 6 | Skeletal Muscle

Fig. 6

From: Six1 homeoprotein drives myofiber type IIA specialization in soleus muscle

Fig. 6

Pvalb is a direct target of Six1. a Schematic representation of the Pvalb promoter. b qPCR values of ChIP experiments performed with Six1 antibodies or IgG on GP and TA chromatin, and showing Six1 binding to Pvalb MEF3_1 and Pvalb MEF3_2. c Competitive Electromobility shift assays performed with recombinant Six1 and Six4 proteins and labeled Myogenin MEF3 oligonucleotide and 10 or 100 fold molar excess of unlabelled oligonucleotides containing Myogenin MEF3 site, NF1 site, or Pvalb MEF3_2 (left panel) and 100-fold molar excess of unlabelled oligonucleotides containing Myogenin MEF3, NF1 site, Myod DRR MEF3 site, or Pvalb MEF3_1 sites (right pannel) whose sequence is presented on a. d Luciferase assays from adult TA muscles electroporated by the indicated luciferase vectors and the TK-renilla luciferase vector allowing normalization. *P < 0.05, ***P < 0.001

Back to article page