Fig. 2
From: Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease
Tamoxifen-induced myogenic reprogramming of iMyoD-transduced UDCs. a RT-PCR time course evaluation of DES (desmin) mRNA expression in two UDC lines (2 and 11). iMyoD-transduced cells were treated with or without tamoxifen (2.5 μM, 24 h) and then cultured in differentiation media as indicated (7, 14, and 28 days). GAPDH mRNA expression is shown as a control. b Real-time semi-quantitative PCR results for DES mRNA expression in urine cell lines 2 and 11. c Immunofluorescence microscopy of MyoD (red) and desmin (green) protein expression in iMyoD-transduced control lines 2 and 11, without tamoxifen treatment. In the uninduced state, MyoD demonstrated a cytoplasmic distribution, and no appreciable desmin expression was observed. Nuclei (blue); Scale bar = 100 μm. d After induction, MyoD (red) became more nuclear concomitant with cytoplasmic desmin (green) protein expression. Myotube elongation progressed over time in culture. Scale bar = 100 μm