Fig. 3
From: Direct reprogramming of urine-derived cells with inducible MyoD for modeling human muscle disease
Myogenic differentiation and maturation of induced iMyoD UDCs. a Timeline for differentiation of iMyoD-transduced cells. b RT-PCR for myogenic maturation markers in iMyoD-transduced line 2, with or without tamoxifen (2.5 μM, 24 h), and culture in differentiation media as indicated (7, 14, and 28 days). Myogenin (MYOG) and embryonic myosin heavy chain (MYH3) were the earliest markers detected. c Real-time semi-quantitative PCR results demonstrated that dystrophin (DMD) and adult myosin heavy chain (MYH2) were detected with further differentiation. d Images of myogenic markers in iMyoD-transduced line 2. Increased muscle marker expression over time was accompanied by myotube elongation. Nuclei (blue); Scale bar = 100 μm