Fig. 10

The number of motor axons and expression of molecules with critical function at the NMJ are unchanged in VAChT^Hyp mice. Cross sections (10 μm) of the common peroneal nerve expressing tdTomato in motor axons were also labeled with antibodies against neurofilament (NF) and the Schwann cell marker, S100, (a). There was no difference found in the number of motor axons (b) or peripheral axons (c) between control and VAChT^Hyp mice at 1 and 5 months of age. The expression of neuronal-agrin isoforms (d) and ChAT (e) are not significantly altered in the spinal cords of 3-month-old VAChT^Hyp mice. At least four male mice maintained on a C57BL/6 background were used per genotype for axonal counts and expression analysis. Scale bar = 50 μm. Error bar = Standard Error