Table 1 PCR primers
Gene | Fw (5′–3′) | Rv (5′–3′) |
|---|---|---|
GAPDH | CCCACTCTTCCACCTTCGATG | GTCCACCACCCTGTTGCTGTAG |
AChE | CTACACCACGGAGGAGAGGA | CTGGTTCTTCCAGTGCACCA |
AChRγ | GCTCAGCTGCAAGTTGATCTC | CCTCCTGCTCCATCTCTGTC |
AChRε | GCTGTGTGGATGCTGTGAAC | GCTGCCCAAAAACAGACATT |
MuSK | CCACACTGCGTGGAATGAGC | CTCTGCAAATGGGCATGGG |
LRP4 | GGCAAAAAGCAGGAACTTGT | TCTACCCAGTGGCCAGAACT |
Rapsyn | GTGCCATGGAGTGTTGTGAG | CGGTTTCCGATCTCAGTCAT |
Dok7 | GGGTACTGGGCTGGAGTCTT | TCGGACGATGCAGTCAAACA |
CDK5 | GCCAGACTATAAGCCCTACCC | GTCAGAGAAGTAGGGGTGCT |
MyHC2A | GAGTGAGCAGAAGCGGAATGCT | GCGGAACTTGGATAGATTTGTG |
MyHC2B | CACCTGGACGATGCTCTCAGA | GCTCTTGCTCGGCCACTCT |
MyHC2X | GCTAGTAACATGGAGGTCA | TAAGGCACTCTTGGCCTTTATC |
Myogenin | GCACTGGAGTTCGGTCCCA | GTGATGCTGTCCACGATGGA |
Foxo1 | GAGTTAGTGAGCAGGCTACATTT | TTGGACTGCTCCTCAGTTCC |
Atrogin-1 | GCAGCAGCTGAATAGCATCCA | GGTGATCGTGAGGCCTTTGAA |
Agrin-8 | CTTTGATGGGCGGACCTACA | CGCTTTCTCAGCTGGGATCT |
Agrin-11 | CAGTGGGGGACCTAGAAACAC | TTTCAGGGCTCTCAGTCACAG |
Agrin-19 | CTTTGATGGGCGGACCTACA | AGTTTCAGGGGCTGGGATCT |
Chat | CCTGGATGGTCCAGGCACT | GTCATACCAACGATTCGCTCC |
VAChT | GAGAGTACTTTGCCTGGGAGGA | GGCCACAGTAAGACCTCCCTTG |