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Fig. 5 | Skeletal Muscle

Fig. 5

From: Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting

Fig. 5

Functional characterization of skeletal muscle progenitor cells isolated by distinct surface marker combinations. a Similar levels of colony formation were seen across cells isolated by β1-integrin and CXCR4, VCam1, and α7-integrin and CD34 sorting paradigms. Data were collected for cells harvested independently from n = 5 mice (two female and three male). Each dot represents colony-forming efficiency of an individual mouse, calculated from analysis of at least 95 wells. Overlay represents mean ± SD. b No differences in myogenic differentiation indices (see “Methods” section) among β1-integrin and CXCR4, VCam1, and α7-integrin and CD34 sorted populations. Data were collected for cells harvested independently from n = 3 female mice. Each dot represents one mouse, with two technical replicates per biological replicate. Overlay indicates mean ± SD. c Representative ×10 images of cultures quantified in (b), derived from sorted β1-integrin and CXCR4 (left), VCam1 (middle), and α7-integrin and CD34 (right) cell populations after 72 h in differentiation media. d Quantification of the percentage of Pax7, MyoD, or MyoG protein expressing cells within myogenic cultures expanded in growth media for 5 days. Error bars represent standard deviations. N = 3, with two technical replicates per biological replicate

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