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Fig. 2 | Skeletal Muscle

Fig. 2

From: Muscle-specific deletion of SOCS3 increases the early inflammatory response but does not affect regeneration after myotoxic injury

Fig. 2

SOCS3 deletion in mature myofibers in vivo does not alter regeneration after myotoxic injury. Control (SOCS3fl/fl MCK-Cre) and SOCS3 MKO (SOCS3fl/fl MCK-Cre+) mice were either left uninjured (UN) or received a single 40 μL injection of notexin (10 μg/ml) into the right TA muscle and were killed for analysis at 1 day (D1), 2 days (D2), 3 days (D3), 5 days (D5), 7 days (D7), or 14 days (D14) post-notexin injury. qRT-PCR using primers to detect MCK (a), Socs3 (b), Pax7 (c), MyoD (d), and Myogenin (e) was performed on RNA extracted from snap frozen muscles following dissection. Data are expressed as mean ± SEM. Statistical analysis was performed using a two-way ANOVA with a Fisher’s LSD post hoc multiple comparisons test to determine the effects of genotype and time. n = 8 mice/time-point/genotype. *P < 0.05, **P < 0.01 compared to control

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