Fig. 3

Fibrosis, necrosis, and M1 macrophages after cardiotoxin injury in mKO mice. a Representative Masson’s trichrome stained section with enlarged area and quantification of fibrotic area in mKO mice 3 weeks after the third CTX injection; n = 4–7 per group; b relative mRNA levels of pro-fibrotic genes at the same time point; CTX-injured muscle gene expression levels were normalized to PBS levels of WT animals; n = 6–7 per group (t test); c representative IHC images (CD68: green; dapi/nuclei: blue; laminin: gray) and corresponding H&E sections showing accumulation of macrophages (green in IHC images) in actively regenerating area (dark purple in H&E staining); d measurement of area occupied by M1 macrophages from IHC images; n = 5 per group; e measurement of necrotic area from H&E sections normalized to total section area; n = 5 per group; values are plotted as average ± SEM; *p ≤ 0.05, ***p ≤ 0.001