Fig 2

SC numbers and proliferation on fibers in culture. a Representative IHC images (DAPI/nuclei: blue; MyoD: green; Pax7: red) of SCs on freshly isolated fibers (T0) and b SC quantification per fiber in mTGs and WT mice. Approximately 60 fibers per mouse and 5 mice per group were used. c Representative IHC images (DAPI/nuclei: blue; MyoD: green; Pax7: red) of SC progeny on fibers kept for 3 days in culture media (T3). Quantification results: d Total proliferative output of SCs. e Normalized proliferative output (T3/T0). f Quantification of Pax7⁺/MyoD⁻, Pax7⁺/MyoD⁺, and Pax7⁻/MyoD⁺ cells per fiber expressed as percentages and g numbers of total proliferative output. Approximately 20 fibers per mouse and 5 mice per group were used. Brightness/contrast was adjusted using ImageJ. Values are plotted as AV ± SEM; *p ≤ 0.05, **p ≤ 0.01