Fig 3
From: Muscle PGC-1α modulates satellite cell number and proliferation by remodeling the stem cell niche
Myoblast proliferation outside of the SC niche. a Assessment of live mTG and WT myoblasts (MBs) 1 and 2 days after plating equal numbers and fold change in MB numbers between these two days. Cells were quantified on 10 fields per dish, 2 dishes per mouse, and 3 mice per genotype. Representative bright-field image with the white rectangle indicating enlarged area. b Desmin-positive cell quantification 2 and 4 days after plating equal number of MBs and fold change between these two days. Cells were quantified on an area of 38.7 mm2 per dish, 2 dishes per mouse, and 3 mice per genotype. Representative IHC image (DAPI/nuclei: red; desmin: green) with the white rectangle indicating enlarged area. Values are plotted as AV ± SEM