Loss of C/EBPβ reduces the proportion of Pax7+ cells in myogenic cultures.
a RT-qPCR analysis of Cebpb expression in primary myoblasts isolated from C/EBPβfl/flPax7+/+ (wild-type) and conditional null C/EBPβ−/−Pax7CreER−/+ (C/EBPβ−/−) subjected to in vitro 4′OH-TAM treatment and cultured in growth medium (GM) (n = 5). b RT-qPCR analysis of Pax7, Myod1, and Myog expression in primary myoblasts isolated and cultured as in a (n = 5). c Representative pictures of myoblasts cultured under growth conditions for 24 h stained for Pax7 (red) and MyoD (green). Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. Percentage of d self-renewing (Pax7+/MyoD−), e differentiating (Pax7−/MyoD+), and f proliferating (Pax7+/MyoD+) WT (black bars) and C/EBPβ−/− (white bars) myoblasts cultured as in a as determined by immunocytochemistry (n = 4). g Representative pictures of cells cultured as in a and immunostained for myogenin (red). The nuclei were counterstained with DAPI (blue). Scale bar, 50 um. h Percentage of myogenin+ cells relative to total nuclei (n = 4). For all panels, data is represented as mean ± SEM, *p < 0.05, ***p < 0.001