Fig. 2

Loss of C/EBPβ inhibits satellite cell self-renewal and promotes differentiation. C/EBPβ^fl/flPax7^+/+ (wild-type) and conditional null C/EBPβ^−/−Pax7^CreER−/+ (C/EBPβ^−/−) mice were injected daily with tamoxifen for 5 days. One week after the last injection, myofibers were isolated from the EDL of each mouse and cultured for 3 days in suspension before immunostaining. a Western analysis of C/EBPβ expression in SCs isolated from the hind limb of WT and C/EBPβ^−/− mice injected with tamoxifen for 5 days. b Representative pictures of myofibers stained for Pax7 (red) and MyoD (green). Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. c Quantification of self-renewing (Pax7⁺/MyoD⁻), proliferating (Pax7⁺/MyoD⁺), and differentiating (Pax7⁻/MyoD⁺) myoblasts on single myofibers (n = 4 mouse pairs). d Representative pictures of myofibers stained for Pax7 (red) and myogenin (green). Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm. e Quantification of myogenin⁺ cells as a percentage of total nuclei (n = 3 mouse pairs). f Total number of cells per cluster (n = 3 mouse pairs). For all panels, data is represented as mean ± SEM, *p < 0.05, **p < 0.01, n.s. not significant