Fig. 3

Loss of C/EBPβ leads to a reduction in reserve cells in vitro. a RT-qPCR analysis of Cebpb expression in primary myoblasts isolated from C/EBPβ conditional knockout mice (C/EBPβ^−/−) or wild-type non-Cre-expressing littermates subjected to in vitro 4’OH-TAM treatment, cultured in GM, and plated and transferred to differentiation media for 48 h (n = 3). b Representative pictures of cells stained for myosin heavy chain (MyHC; green) and Pax7 (red). Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. c Percentage of Pax7⁺ cells relative to total nuclei (n = 3). d Reserve cells were collected and counted following limited trypsinization after 48 h in differentiation media. After counting, the reserve cells were expanded, re-plated at equal densities, and induced to differentiate in low serum for another 48 h. The number of reserve cells was counted after each round of differentiation and represented as a percentage of total nuclei (n = 3). Data is represented as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001