Fig. 5
From: CCAAT/enhancer binding protein β is required for satellite cell self-renewal
Loss of C/EBPβ expression in satellite cells decreases regenerative capacity following serial injury. a Experimental design. C/EBPβfl/fl Pax7+/+ (wild-type) and conditional null C/EBPβ−/−Pax7CreER−/+ (C/EBP β−/−) mice were injected daily with tamoxifen for 5 days. One week after the last injection, the left tibialis anterior (TA) muscle was injured using 50 μl of 1.2% BaCl2 (1st injury). Twenty-one days after the initial injury, the same TA muscle was injured again using 50 μl of 1.2% BaCl2 (2nd injury). The muscle was analyzed at 7 and 21 days after the 2nd injury (dpi). b Cross-sections of injured TA 7 dpi were stained with embryonic myosin heavy chain (eMyHC; red) and laminin (LAM; green). Nuclei were counterstained with DAPI (blue). Representative pictures are shown. Scale bar: 50 μm. c Average cross-sectional area of eMyHC+ fibers from TA sections stained as in b (n = 5 mouse pairs). d Pax7+ nuclei were counted in 7 dpi muscle and represented as total Pax7+ cells per area (mm2) (n = 4 mouse pairs). e Cross-sections of injured TA 21 dpi were stained for hematoxylin (nuclei; purple) and eosin (pink) (H&E). Representative pictures are shown. Scale bar, 50 μm. f Average cross-sectional area of myofibers from TA sections stained as in e (n = 6 mouse pairs). g Pax7+ nuclei were counted in 21 dpi muscle and represented as total Pax7+ cells per area (mm2) (n = 3 mouse pairs). Data is represented as mean ± SEM, *p < 0.05, **p < 0.01