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Fig. 6 | Skeletal Muscle

Fig. 6

From: Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4

Fig. 6

SC35 speckles in most nuclei were disrupted by exogenous DUX4-FL expression. a–c BacMam-mediated expression of DUX4-FL (green) in healthy control myotubes caused SC35 speckles (red) to show an altered morphology. Arrows indicate nuclei that expressed DUX4-FL, and asterisks indicate nearby nuclei that were DUX4-FL-negative. SC35 speckles in the DUX4-FL-positive nuclei typically showed larger aggregates and/or more intense staining. SC35 speckles were disrupted in both nuclei with punctate DUX4-FL staining (rows a, c) and in nuclei with uniform DUX4-FL staining (row b). In nuclei with punctate DUX4-FL (rows a, c), there was little or no overlap between DUX4-FL and SC35 staining. d, e In contrast to expression of DUX4-FL, exogenous, BacMam-mediated expression of DUX4-S (row d) or PITX1 (row e) did not markedly affect SC35 speckles in most nuclei. See Fig. 7e for quantitation of the extent to which exogenous DUX4-FL, DUX4-S, and PITX1 affects SC35 speckles using blind assays. Bar in b = 20 μm for a, b, d, and e and 12 μm for c

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