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Fig. 8 | Skeletal Muscle

Fig. 8

From: Nuclear bodies reorganize during myogenesis in vitro and are differentially disrupted by expression of FSHD-associated DUX4

Fig. 8

Exogenous DUX4-FL expression induced abnormalities in FUS expression. At 24 h after addition of BacMam vector, healthy control myotubes were examined for expression of exogenous DUX4-FL (red) and FUS (green). a–c” About 40–50% of the DUX4-FL-positive myotube nuclei showed punctate immunostaining for FUS (panels ac). In addition, DUX4-FL itself showed punctate staining in some nuclei and merged images indicated that FUS and DUX4-FL puncta were not usually co-localized. d–d” About 10% of the DUX4-FL-positive myotube nuclei showed little or no staining for FUS (panel d). e–e” The remaining approximately half of the DUX4-FL-positive myotube nuclei showed a more uniform distribution of FUS staining in the nucleus (though excluded from nucleoli), even when DUX4-FL staining was itself punctate. fi For comparison, myotube nuclei that did not express DUX4-FL typically showed the more uniform pattern of FUS staining (green) without large puncta, which was similar to the nucleus in panel e. Bar in a = 10 μm for ae” and 8 μm for fi

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