Fig. 9

Exogenous DUX4-FL expression induced nuclear co-aggregates of FUS and TDP-43. a-a” Double immunostaining of TDP-43 (red) and FUS (green) in myotubes which also expressed exogenous DUX4-FL showed that TDP-43 and FUS staining were almost completely co-localized. b–b” In rare myotube nuclei, some TDP-43 puncta (red arrows) and FUS puncta (green arrows) did not co-localize with the other protein, even though most of the FUS and TDP-43 in the same nucleus were co-localized. Bar in a = 10 μm