Fig. 3

HO-1 expression is reduced in dystrophic muscle, and PGC-1α rescues HO-1 expression. a Relative gene expression of the indicated genes in the indicated muscle beds from transgenic mice expressing PGC-1α in the skeletal muscle (MCK-PGC-1a), versus controls. b Relative gene expression of the indicated genes in myotubes expressing adenovirus-driven PGC-1α (ad_PGC-1a), versus GFP controls. c, d Relative gene expression (c) and protein expression (d) of HO-1 in the muscle from mdx mice, versus controls. Quantification is shown on the right. e Relative gene expression of the indicated genes in the muscle from patients with DMD, versus matched controls. N = 3 per group. f, g Relative gene expression (f) and protein expression (g) of HO-1 in the muscle from mdx/MCK-PGC1a and mdx/MCK-PGC-1b transgenic mice, versus mdx alone. Quantification of protein expression is shown on the right. n = 5. Red bars highlight HMOX1 data. *P < 0.05, Student’s t test. Error bars indicate ±SEM