Fig. 3

Heatmap showing the expression levels in immortalized and primary myogenic human lines of genes that are consistently and strongly downregulated in a panel of previous studies of myoblast differentiation. A gene is shown if it was among the 300 most strongly downregulated after five or more days of differentiation in at least three of four studies of human and mouse primary myoblasts, and C2C12 myoblasts. Expression values are row scaled (i.e., changes are relative across each row for ease of interpretation). Hierarchical clustering analysis of the cell lines, indicated by branches at top, places myotube lines (indicated in lilac in the cell_type bar at top) separately from myoblasts (cyan cell_type), except for the C25 primary cells (green cell_type). The non-myogenic population (orange cell_type) clusters with the myotubes. Immortalized clones (green clonal_state) show the same switch from myoblast to myotube as their parent primary populations (yellow clonal_state). Hierarchical clustering analysis was also applied to genes (branches to left)