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Fig. 2 | Skeletal Muscle

Fig. 2

From: Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Fig. 2

Laminin 521 is the only substrate capable of maintaining differentiation after eight passages in Dba satellite cell-derived myoblasts. a Quantification of differentiation via MHC area calculation. b Quantification of total nuclei per well via Hoechst nuclei count. Statistical significance was determined by one-way ANOVA with Bonferroni correction. N = 3 technical replicates per group from one biological sample. Significance is annotated as less than .01 (**), and less than .0001(****). All comparisons were conducted using laminin 521 as control. c Differentiation index, calculation of proportion of MHC+ myotubes containing two or more nuclei, MHC+ myocytes containing a single nuclei, and proportion of MHC− cells. d Immunostaining for MHC (green) and Hoechst (blue). Scale bar represents 200 μM. N = 4 wells per group. e, f Multi-nucleation index quantifying the number of nuclei per myotube as a proportion of total nuclei at passage 6 (e) and passage 8 (f). Statistical significance for e, f was determined by linear regression. The distribution of laminin 521 was found to be significantly different, p < .001, compared to laminin 111 and MG

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