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Fig. 3 | Skeletal Muscle

Fig. 3

From: Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion

Fig. 3

Laminin 521 supports robust differentiation in C57/BL6 and C57/BL10 backgrounds after eight passages. Quantification of differentiation via MHC area calculation in proportion to total nuclei for BL6 (a) and BL10 (b). c, d Multi-nucleation index quantifying the number of nuclei per myotube as a proportion of total nuclei in BL6 (c) and BL10 (d) cultures at passage 8. Statistical significance for a, b was calculated using one-way ANOVA with Bonferroni correction. N = 3 technical replicates per group. Significance is annotated as less than .001 (***). All comparisons were conducted using laminin 521 as control. Statistical significance for c, d was calculated using linear regression. The distribution of laminin 521 was found to be significantly different, p < .001, compared to laminin 111 and MG

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Skeletal Muscle

ISSN: 2044-5040

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