Fig. 2

Average distances run by male and female mice per hour over 24 h, over 34 weeks of RWE. Graphs (a–g) depict average running distances by male and female mice at each level of resistance (0–6 g) throughout the exercise protocol: weeks 1 and 2 with resistance set at 0 g (a); weeks 3 and 4 (1 g) (b); weeks 5 and 6 (2 g) (c); weeks 7 and 8 (3 g) (d); weeks 9 to 12 (4 g) (e); weeks 13 to 20 (5 g) (f); and weeks 21 to 34 (6 g) (g). N = 6–7 mice per group. Gray and white bars under the X-axis indicate scotophase (1900–0700 h) and photophase (0700–1900 h). Error bars were omitted for clarity. Asterisk denotes a significance between males and females; *P < 0.05; **P < 0.01; ***P < 0.001