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Fig. 1 | Skeletal Muscle

Fig. 1

From: A novel method for the quantification of fatty infiltration in skeletal muscle

Fig. 1

Illustration of qualitative inspection of fatty infiltration by decellularization and oil red O (ORO) staining. a A representative isolated 5th toe EDL muscle. b The same muscle following decellularization. Decellularization removes myocellular proteins but spares large lipid droplets visible as spherical structure with increased reflectance in a semi-transparent construct. c The same muscle following staining with the lipid soluble dye ORO where lipid droplets are stained bright red. Some superficial lipid is visible in the intact muscle and can be tracked through the decellularization and staining process (inset, using the proximal vessel as an anatomical landmark). Scale bars are 500 μm

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