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Fig. 2 | Skeletal Muscle

Fig. 2

From: A novel method for the quantification of fatty infiltration in skeletal muscle

Fig. 2

Fatty infiltrate lipid, but not intramyocellular lipid, is retained in decellularized muscles. a An isolated 5th toe EDL muscle stained with osmium tetroxide. The opaque black appearance of the muscle is due to osmium binding with intramyocellular lipid and phospholipids in fiber membranes in addition to fatty infiltrate lipid. b 3D rendering of the osmium signal acquired via μCT. Thresholding was used to isolate the high signal intensity originating in fatty infiltrate from the low signal originating from myocellular lipids. c Decellularization of the same muscle following imaging. Retained lipid, stained black, has the same qualitative distribution pattern as the 3D μCT rendering. d Re-imaging of the decellularized muscle yields a similar 3D μCT rendering to that obtained from the intact muscle. Note that the shift in orientation in the lower quadrant is the result of bending during re-embedding in agarose. e Triglyeride content in tibialis anterior (TA) muscles quantified by lipid extraction and normalized to pre-decellularized muscle weight. Both intact and decellularized muscles treated with GLY have significantly higher triglyceride content than intact and decellularized muscles treated with SAL, respectively. Scale bars are 500 μm. In the SAL treatment group, nearly all lipids present in the intact group is eliminated in the decellularized group. ***p < 0.0005

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