Fig. 3
From: A novel method for the quantification of fatty infiltration in skeletal muscle
Illustration of quantitative analysis of lipid droplet metrics by fluorescent confocal microscopy. a ORO (red)-stained decellularized 5th toe EDL muscle used for qualitative reference. Scale bar is 500 μm. b Two representative image slices from a confocal stack acquired of the image area boxed in panel a. BODIPY-positive (green) circular areas can be clearly differentiated. Some of the same areas can be seen in both slices (asterisks and arrows) but have maximal signal strength only in one (the slice where the ROI was registered). c The 3D reconstruction performed on all registered ROIs. Qualitative comparison of this map and the ORO-stained image in panel a shows good representation through the muscle volume