Fig. 1

Skeletal muscle deletion of SLK. a Schematic representation of the SLK locus and the SLK-targeted allele showing the Frt (black arrowhead) and loxP sites (grey arrowhead). The location of SLK primers are indicated in the Flp-recombined and Cre-recombined alleles. b PCR genotyping of genomic DNA from ear clips for the SLK +/+, SLK +/fl, and SLK fl/fl alleles using 3′F and 3′R primers. c Genomic DNA PCR on the heart and skeletal muscle from wild type and knockout mice using the three SLK primers. Recombination product is only observed following Cre-mediated deletion (500 bp). The 3′F and 3′R primers detect non-recombined DNA strand (471 bp). The 5′F and 3′R only generate a product following recombination, whereas the non-recombined allele (>5 Kbp) could not be amplified by the 5′F and 3′R primers. d Western blot on adult skeletal muscle lysates from wild type and knockout mice. The membrane was probed with an anti-SLK and anti-GAPDH as a loading control. SLK staining on TA muscles from e wild type and f knockout mice. Prominent staining in fibers from wild type (indicated with white arrows) which is not present in knockout. g Western blot analysis of SLK expression in primary cultures of Myf5-Cre:SLK^fl/fl myoblasts showing a marked reduction in SLK levels. h Total levels of SLK protein within different tissues showing lower expression in normal skeletal muscles