Fig. 6

SLK-deficient muscles display a delayed regenerative response. Primary myoblasts isolated from SLK^fl/fl mice were treated with AdLacz or AdCre and then differentiated. After 4 days, the cultures were stained with MF-20 (a, b) or Myogenin (c, d) and myoblast fusion was assessed (AdLacZ 45.1%, AdCre 10.7%, p < 0.01). e–j The TA muscles of 10–12-week-old mice were injected with cardiotoxin and muscle regeneration was assessed by measuring fiber area. SLK-deficient mice showed a reduction in caliber size 7 days post-injection that was no longer apparent at 21 days, suggesting a delayed response (n = 5/genotype). Seven-day regenerates were also stained for Pax7 (k–m) and Myf5 (m–p). No changes in Pax7+ or Myf5+ nuclei were observed between the groups (n = 3/genotype)