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Fig. 3 | Skeletal Muscle

Fig. 3

From: Inhibition of Stat3 signaling ameliorates atrophy of the soleus muscles in mice lacking the vitamin D receptor

Fig. 3

VDR−/− soleus but not TA muscles display increased IL-6 protein levels. a Serum IL-6, IFNγ, and TNFα levels were measured by ELISA in triplicate wells from 8-week-old WT and V mice. Graph shows that no significant differences in cytokine levels were observed in the serum from the two sets (n = 5). b, c Cytokine levels in a were measured in the soleus (b) and TA (c) muscle lysates isolated from 8-week-old WT and V mice. Only IL-6 levels were observed to be significantly increased in the soleus muscles of V mice (n = 5) (**p < 0.01). Cytokine levels were normalized to tissue weight (mgs). d Top and bottom panels show a representative western blot analysis of lysates from the soleus and TA muscles, respectively, of WT and V mice probed with IL-6 antibody. Graph to the right shows the quantitative differences between the two groups. IL-6 protein levels are increased in the soleus but not in the TA muscles of V compared to WT mice. GAPDH serves as the loading control (n = 6)

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