Fig. 2

Electric stimulation eliminated the effects of denervation on the mitoflash activity. The representative confocal images of muscle fibers isolated from the denervated FDB muscle before field stimulation (Den) (a1–3), 10 s after the field stimulation (a4–6), and 120 s after the field stimulation (a7–9). Panels a1, a4, and a7 show the initial fluorescence images at t = 0 s. Panels a2, a5, and a8 show the peak intensity map of all mitoflashes detected in 100 recorded images. Panels a3, a6, and a9 are the 3D surface plots of (a2, a5, and a8). The representative confocal images of muscle fibers isolated from the sham FDB muscle before field stimulation (sham) (b1–3), 10 s after the field stimulation (b4–6), and 120 s after the field stimulation (b7–9). Panels b1, b4, and b7 show the initial fluorescence images at t = 0 s. Panels b2, b5, and b8 show the peak intensity map of all mitoflashes detected in 100 recorded images. Panels b3, b6, and b9 are the 3D surface plots of (b2, b5, and b8). The quantification of the mitoflashes are illustrated in c Total Flash Area/Fiber Area, d FAHM, and e the signal amplitude