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Fig. 3 | Skeletal Muscle

Fig. 3

From: Absence of physiological Ca2+ transients is an initial trigger for mitochondrial dysfunction in skeletal muscle following denervation

Fig. 3

Ru360 eliminated the effect of field stimulation on mitoflash activity. The representative confocal images of muscle fibers isolated from the denervated FDB muscle fibers before field stimulation (Den a1–3), and 120 s after the field stimulation (a4–6) in the presence of 10 μM Ru360. Panels a1 and a4 show the initial fluorescence images at t = 0 s. Panels a2 and a5 show the peak intensity map of all mitoflashes detected in 100 recorded images. Panels a3 and a6 are the 3D surface plots of (a2 and a5). The representative confocal images of muscle fibers isolated from the sham FDB muscle before the field stimulation (sham) (b1–3), 120 s after the field stimulation (b4–6). Panels b1 and b4 show the initial fluorescence images at t = 0 s. Panels b2 and b5 show the peak intensity map of all mitoflashes detected in 100 recorded images. Panels b3 and b6 are the 3D surface plots of (b2 and b5) in the presence of 10 μM Ru360. The quantification of the Total Flash Area/Fiber Area of the mitoflashes is summarized in (c). Note that in the presence of Ru360, there are no significant changes in mitoflash activity before and after the field stimulation (n = 5, p > 0.05)

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