Fig. 6

Mitochondrial Ca²⁺ transient synchronized the time course of the cytosolic Ca²⁺ transient during an elongated depolarization pulse. a The representative confocal images of cytosolic x-rhod-1 and mitochondrial mt-YC3.6 during this 800 ms depolarization (from eight independent experiments). b The time course of cytosolic x-rhod-1 (red trace) and mt-YC3.6 ratio (black trace) during a 800 ms depolarization pulse. c The time course of quantified Ca²⁺ transients in cytosol (red) and mitochondria (black). Although the mitochondrial Ca²⁺ transient shows a delay to reach the peak value, the time course of mitochondrial Ca²⁺ transient did synchronize with the cytosolic Ca²⁺ transient