Fig. 5

Lentiviral transduction decreases myogenic activity in primary mouse myoblasts. a. Representative 20× IF images for Hoechst (blue), Ki67 (red), and Pax7 (green). Percent of proliferative myogenic cells Ki67+/Pax7+ were quantified out of total Hoechst + cell numbers and were found to be significantly decreased after transduced with either empty vector particles or non-target GFP shRNA vectors. b. Number of single positive (Pax7+ or Ki67+) cells out of total Hoechst + cells were quantified and each population was diminished by either empty vector particles or non-target GFP shRNA vectors. (N = 3, ** P ≤ 0.01 ***P ≤ 0.001)