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Fig. 3 | Skeletal Muscle

Fig. 3

From: Retinoid acid-induced microRNA-31-5p suppresses myogenic proliferation and differentiation by targeting CamkIIδ

Fig. 3

The effects on C2C12 cells proliferation by loss-of-function of miR-31-5p. a The RNA levels of miR-31-5p and CamkIIδ after 24 h transfection of miR-31-5p inhibitor and inhibitor NC, qRT-PCR indicated that the expression level of miR-31-5p was down-regulated significantly, but the CamkIIδ level was elevated dramatically when treated with or without RA. b Western blotting confirmed that CaMKIIδ was up-regulated significantly by the inhibitor in absence or presence of RA (1.35- and 1.10-folds, respectively). cd The impacts of miR-31-5p inhibitor on the proliferation of C2C12 cells. CCK-8 assay indicated that the inhibition of miR-31-5p promoted cell proliferation and partially rescued the disturb proliferation induced by RA (c). Edu staining assay and statistical analysis showed that the proliferation ratio of C2C12 cells increased no matter with or without RA (d)

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