Fig. 6

Inhibition of miR-31-5p promoted C2C12 cells differentiation. a qRT-PCR assay for expressions of CamkIIδ, MyoD, and Myogenin in differentiation of C2C12 cells after miR-31-5p inhibition. The relative expression levels of CamkIIδ, MyoD, and Myogenin were up-regulated without RA treatment, and in addition of RA, the MyoD was immune to the influence, but the levels of CamkIIδ and Myogenin were elevated. b Immunofluorescence of myosin and statistical analysis affected by miR-31-5p inhibition. MiR-31-5p inhibition promoted cell differentiation and myosin expression with or without RA. The fusion index confirmed the promotion of myotubes by miR-31-5p inhibitor. c Western blotting assay for detection of the effects of miR-31-5p inhibition on C2C12 cells differentiation. In presence of RA, the relative protein of myosin and CaMKIIδ were decreased, both of the two were remarkably up-regulated with miR-31-5p inhibitor than those without inhibitor. (*P < 0.05 and **P < 0.01)