Fig. 7

Knock-down of CamkIIδ repressed the proliferation and myogenic differentiation of C2C12 cells. a, b Valid knock-down effects of CamkIIδ by transfection of siCamkIIδ for 24 h. qRT-PCR (a) and Western blotting analysis (b) showed that the expression of CamkIIδ mRNA and protein were down-regulated 56.3 and 29.2%, respectively, after siCamkIIδ transfection for 24 and 48 h, respectively. c, d siCamkIIδinhibited the proliferation of C2C12 cells. CCK-8 assay showed that after 48 h transfection of siCamkIIδ, the number of C2C12 cells increased much slower than in control group (c). Edu assay indicated that transfection of siCamkIIδresulted in a decrease of Edu labeling cells to 60% of control (d). e, g siCamkIIδ impaired the myogenic differentiation of C2C12 cells after 4-day transfection. e qRT-PCR indicated the significant decrease of CamkIIδ, MyoD, and Myogenin by siCaMKIIδ transfection. f Western blotting analysis showed the dramatic down-regulation of myosin and CaMKIIδ in siCamkIIδ transfection group. g Immunofluresence of myosin staining indicated the myosin expression and fusion index reduces in siCamkIIδ inhibition group. (*P < 0.05 and **P < 0.01)