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Fig. 3 | Skeletal Muscle

Fig. 3

From: Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F

Fig. 3

Expression of nSPN protein in skeletal muscle from multiple sources. Immunoblots containing BSA alone, BSA coupled to nSPN (BSA-nSPN), and BSA coupled to nSPN-specific Peptide 20 (BSA-nSPN) (a). R20 antibodies recognized an ~11-kDa protein band on immunoblots of total microsomes prepared from mouse and rabbit skeletal muscles (bd). Binding of R20 antibodies (R20 Ab) to nSPN was inhibited by nSPN peptides (c), but not by non-specific control peptides (d). Nitrocellulose membranes of protein lysates from wild-type (non-transgenic) and μSPN-transgenic (Tg) skeletal muscle were probed with μSPN-specific antibodies (R12), which demonstrate cross-reactivity to μSPN-transgenic protein as expected (e). However, nSPN antibodies do not cross-react with μSPN (e). Total protein lysates from wild-type (non-transgenic) and SSPN-Tg skeletal muscle were probed with SSPN-specific antibodies (R15) that cross-react with SSPN, as expected (f). However, nSPN antibodies (R20) do not cross-react with SSPN (e). Table 2 summarizes antibodies used in the study. Molecular weight markers (kDa) are provided on the left

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