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Fig. 4 | Skeletal Muscle

Fig. 4

From: Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F

Fig. 4

Analysis of nSPN in isolated T-tubule and sarcoplasmic reticulum membranes. T-tubule sarcoplasmic reticulum (SR) membranes were prepared from skeletal muscle from control wild-type (WT) and δ-SG-null (Sgcd) mice. Samples analyzed include T-tubules (a), light SR (b), and heavy SR (c). Immunoblotting of DHPR, SERCA1, and RyR in different fractions of muscle microsomes after sucrose gradient centrifugation to separate T-tubule and SR fractions. Previous studies have shown that γ-SG, δ-SG, δ-SG3, and μSPN are present in the light SR of control mice, but are absent in the light SR of δ-SG-null mice [26]. Additionally, our previous analysis using identical preparations demonstrated δ-SG3 and μSPN are enriched in the heavy SR of wild-type muscle, but are lost in the heavy SR of the δ-SG-null mice. Molecular weight markers (kDa) are provided on the left

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Skeletal Muscle

ISSN: 2044-5040

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