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Fig. 5 | Skeletal Muscle

Fig. 5

From: Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F

Fig. 5

Nanospan localization is consistent with triad localization in mildly stretched skeletal muscle fibers. TPLSM study of nSPN localization in stretched FDB skeletal muscle fibers from wild-type (C57-BL/6J) mice. a, b TPLSM images of an FDB muscle simultaneously labeled with nSPN (Alexa 488) and α-actinin (Texas Red) antibodies, respectively. c The superposition of the images on a and b. d, e Simultaneous TPLSM images of immunolocalization of nSPN and the SHG obtained from an FDB muscle labeled with an antibody against nSPN (Alexa 488), respectively. f The superposition of the images on d and e. g The superposition of the Alexa 488 (nSPN, green trace) and Texas Red (α-actinin, red trace) fluorescence plot profiles of the fibers shown in a–c. h The superposition of the Alexa 488 emission (nSPN, green trace) and SHG (blue trace) profiles of the fibers shown in d–f (profiles taken from areas indicated with boxes). The schematic diagrams at the bottom of g and h show the correspondence of the intensity profiles with main sarcomeric hallmarks. Sarcomeric length of the fibers presented in this figure is ~3 μm. Scale bar, 10 μm

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Skeletal Muscle

ISSN: 2044-5040

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