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Fig. 6 | Skeletal Muscle

Fig. 6

From: Nanospan, an alternatively spliced isoform of sarcospan, localizes to the sarcoplasmic reticulum in skeletal muscle and is absent in limb girdle muscular dystrophy 2F

Fig. 6

Immunolocalization of muscle proteins using two-photon laser scanning microscopy. Two-photon laser scanning microscopy (TPLSM) was used to study the localization of DHPR, RYR-1, SERCA, and IP3R expression in skeletal muscle fibers from wild-type (C57-BL/6J) mice. b, e, h, k TPLSM images of the SHG signals obtained simultaneously to the fluorescent signal from fibers labeled with antibodies against SERCA (a, Texas Red), IP3R (d, Texas Red), DHPR (g, Texas Red), and RyR-1 (j, Texas Red). c, f, i, l The superposition of the images of the Texas Red fluorescence and SHG signals obtained from the different muscles. m The superposition of the Texas Red and SHG fluorescence plot profiles of the fiber shown in jl and the relative localization of the RyR-1 expression in the sarcomere. n The superposition of the Texas Red and SHG fluorescence plot profiles of the fiber shown in df and the relative localization of the IP3R expression in the sarcomere. Scale bar, 10 μm

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