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Fig. 2 | Skeletal Muscle

Fig. 2

From: SMCHD1 regulates a limited set of gene clusters on autosomal chromosomes

Fig. 2

SMCHD1 binding to the PCDH cluster control region and transcriptional expression of the PCDHβ cluster. qPCR analysis on SMCHD1 ChIPed DNA for HS regions known to control the expression of PCDHα, (a) HS5-1 and PCDHβ, (b) HS17-17′and HS19-20, and cluster isoforms. Results represent the log10 input normalized binding enrichment relative to IgG, which has been arbitrarily set to 1. Asterisks directly over bars represent a significant increase in binding relative to IgG; asterisks on a line indicate a significant difference in binding between cell types. *Bonferroni adjusted P < 0.05, t test; n = 4 for controls, n = 2 for FSHD1, n = 5 for FSHD2; error bars = SEM. RNA expression analysis of PCDHβ cluster isoform members in primary control, FSHD1, and FSHD2 (c) myoblast and (d) myotube cells. Results represent qRT-PCR analysis of the indicated gene after normalization to the internal control gene GUS1. For each gene, the log10 value of expression is given relative to control individuals where this value was arbitrarily set to 1. *Bonferroni adjusted P < 0.05, t test; n = 4 for controls, n = 10 for FSHD1, n = 7 for FSHD2; error bars = SEM

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